Effects of herbal-based toothpastes on cell viability

Purpose: To evaluate the effects of herbal-based toothpastes with different contents on viability L929 cells.Methods: Herbal-based {Aloe vera - propolis herbal toothpaste, Group 1; Parodontax, 2; Toothpaste miswak, and tea tree extract, 3, Dent natural protective toothpaste clay, 4; Gano fresh, 5} were diluted (1:1, 1:2, 1:4, 1:8, 1:16, 1:32) in Dulbecco’s modified Eagle’s medium. fibroblast cells treated medium containing for 2 min. Cell was assessed using methyl tetrazolium test. The negative control group set at 100 %, percentage all groups determined accordingly.Results: significantly reduced dilutions 5 (p = 0.00). This trend also observed 4. All except 1:32 affected cell In 2, only 1:1 dilution showed a toxic effect samples Groups 1 3 did not show statistically significant cytotoxicity to > 0.05).Conclusion: Herbal substances such as sodium lauryl sulfate (Group 5) benzoate (Groups 4) are cytotoxic towards cells. contain detergents but potassium sorbate preservative; hence, they toxic.